Abstract
Triiodothyronine (T3) is known to increase liver lipogenic enzyme gene expression both in vivo and in tissue culture. Conflicting results have been reported on the effect of T3 on lipogenic enzyme gene expression in white adipose tissue. The results presented
in this paper indicate that administration of pharmacological doses of T3 in rats leads to increased fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC),
ATP-citrate lyase (ACL) and malic enzyme (ME) activity in white adipose tissue. The
increase in lipogenic enzyme activity was associated with increased FAS, ACC, ACL
and ME mRNA levels. The response was dose-dependent. Activity of lipogenic enzyme
and the lipogenic enzyme mRNA levels were positively correlated to serum T3 concentration. The in vivo effect of T3 on lipogenic enzyme gene expression could be reproduced in primary white rat adipocyte
culture. In conclusion, the results presented in this paper indicate that T3 exerts a stimulatory effect on lipogenic enzyme gene expression in white adipose
tissue both in vivo and in tissue culture. Significant effects of T3 on lipogenic enzyme gene expression were only observed in the presence of relatively
high (pharmacological) concentrations of the hormone.
Key words
Triiodothyronine - lipogenic enzyme activity - mRNA - white adipose tissue - rat adipocytes
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Julian Swierczynski
Department of Biochemistry · Medical University of Gdansk
Debinki 1 · 80-211 Gdansk · Poland
Phone: +48 (58) 349 14 62
Fax: +48 (58) 349 14 65
Email: juls@amg.gda.pl
